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Goat Anti-BMP1 (aa284-296) Antibody (EB12632)

Related Products: EBP12632 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12632 Goat Anti-BMP1 (aa284-296) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12632 1
EB12632 (1ug/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12632 2
EB12632 (2ug/ml) staining of Jurkat (A) and (1ug/ml) A549 (B), MCF7 (C) HeLa (D), NIH3T3 (E) and PC12 (F) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12632 3
EB12632 (6µg/ml) staining of paraffin embedded Human Colon. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB12632 4
EB12632 Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.
EB12632 5
EB12632 (6µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB12632 6
EB12632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BMP1, bone morphogenetic protein 1, OI13, PCOLC, PCP, PCP2, TLD, mammalian tolloid protein, procollagen C-endopeptidase, procollagen C-proteinase
Official Symbol: BMP1
Accession Number(s): NP_001190.1; NP_006120.1
Human GeneID(s): 649
Non-Human GeneID(s): 12153 (mouse), 83470 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).

Immunogen

Peptide with sequence C-DTIVPKYEVNGVK, from the internal region of the protein sequence according to NP_001190.1; NP_006120.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75-80kDa band observed in Human Liver lysates and in lysates of cell. lines HeLa, Jurkat, MCF7, NIH3T3 and PC12 (calculated MW of 82.9kDa according to Human NP_001190.1 and, 93.5kDa according to Mouse NP_001409046.1, and Rat XP_006252347.1). This molecular weight is observed by other sources and was successfully blocked by incubation with the immunizing peptide, corresponding to the cleaved BMP-1 propeptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Colon and Placenta. Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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