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Goat Anti-CAPRIN1 Antibody (EB12638)

Related Products: EBP12638 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12638 Goat Anti-CAPRIN1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12638 1
EB12638 (0.3µg/ml) staining of Jurkat (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12638 2
EB12638 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAPRIN1, cell cycle associated protein 1, GPIAP1, GPIP137, M11S1, RNG105, p137GPI, GPI-anchored membrane protein 1, GPI-anchored protein p137, GPI-p137, RNA granule protein 105, activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle
Official Symbol: CAPRIN1
Accession Number(s): NP_005889.3; NP_976240.1
Human GeneID(s): 4076
Non-Human GeneID(s): 362173 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1).

Immunogen

Peptide with sequence C-EQRPQKEPIDQ, from the internal region of the protein sequence according to NP_005889.3; NP_976240.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80+140kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4kDa according to NP_005889.3). The 140kDa band corresponds to the reported p137 (Ellis and Luzio,.PMID: 7657653). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hoiur at room temperature.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Pig

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