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Goat Anti-CD68 Antibody (EB12699)

Related Products: EBP12699 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12699 Goat Anti-CD68 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12699 1
EB12699 (2µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12699 2
EB12699 (5µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB12699 3
EB12699 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
EB12699 4
EB12699 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD68, CD68 molecule, GP110, LAMP4, SCARD1, CD68 antigen, macrophage antigen CD68, macrosialin, scavenger receptor class D, member 1
Official Symbol: CD68
Accession Number(s): NP_001242.2; NP_001035148.1
Human GeneID(s): 968
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001242.2; NP_001035148.1).

Immunogen

Peptide with sequence PNKTKVQGSCEGAH, from the internal region of the protein sequence according to NP_001242.2; NP_001035148.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 100-110kDa band observed in lysates of cell line U937 and in preliminary testing of THP-1 cell lysate (calculated MW of 37.4kDa according to NP_001242.2). The observed molecular weight is routinely reported, and corresponds to the glycosylated form. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5-6µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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