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Goat Anti-Ketohexokinase Antibody (EB12963)

Related Products: EBP12963 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12963 Goat Anti-Ketohexokinase Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $423.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12963 1
EB12963 (0.03µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Liver lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12963 2
EB12963 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: KHK, ketohexokinase (fructokinase), hepatic fructokinase, ketohexokinase
Official Symbol: KHK
Accession Number(s): NP_000212.1; NP_006479.1
Human GeneID(s): 3795
Non-Human GeneID(s): 16548 (mouse), 25659 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000212.1; NP_006479.1).

Immunogen

Peptide with sequence RCLSQRWQRGGNASN , from the internal region of the protein sequence according to NP_000212.1; NP_006479.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 30kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 32.7kDa according to Human NP_000212.1, Mouse NP_032465.2, Rat NP_114061.1 and of 32.6kDa according to Pig XP_003125348.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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