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Goat Anti-Nsg2 (mouse, C terminus) Antibody (EB12967)

Related Products: EBP12967 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12967 Goat Anti-Nsg2 (mouse, C terminus) Antibody Pep-ELISA, WB, ICC Yes 100µg specific antibody in 200µl Mouse, Rat $423.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12967 1
EB12967 (0.1µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Nsg2, neuron specific gene family member 2, protein 8.5, protein p19
Official Symbol: Nsg2
Accession Number(s): NP_032767.1
Non-Human GeneID(s): 18197 (mouse)
Important Comments: Reported variants represent identical protein: NP_001277610.1, NP_001277609.1, NP_032767.1

Immunogen

Peptide with sequence C-HEPKPPKTQGH, from the C Terminus of the protein sequence according to NP_032767.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 19kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0kDa according to NP_032767.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat: Chan Choo Yap et al. (2017) PMID: 28874679.

Immunocytochemistry: This antibody has been successfully used in ICC on Rat: Chan Choo Yap et al. (2022) PMID: 35474277.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse

Specific References

This antibody has been successfully used in ICC on Rat:
Chan Choo Yap, Laura Digilio, Lloyd P McMahon, Tuanlao Wang, Bettina Winckler
Dynein is required for Rab7-dependent endosome maturation, retrograde dendritic transport, and degradation
J Neurosci. 2022 Apr 26;JN-RM-2530-21.
PMID: 35474277

This antibody has been successfully used in Western blot on Rat:
Chan Choo Yap, Laura Digilio, Lloyd McMahon & Bettina Winckler
The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.
Scientific Reports, 7: 10481, DOI: 10.1038/s41598-017-07667-x (2017)
PMID: 28874679

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