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Goat Anti-4E-T / EIF4ENIF1 Antibody (EB06230)

Related Products: EBP06230 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06230 Goat Anti-4E-T / EIF4ENIF1 Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $300.00 Elite

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San Diego
CA 92121-4727
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Toll Free Tel: 1-800-550-3033
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EB06230 1
EB06230 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06230 2
EB06230 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000063276, FLJ26551, eIF4E-transporter, eukaryotic translation initiation factor 4E nuclear import factor 1, 2610509L04Rik, FLJ21601, Clast4, 4E-T, EIF4ENIF1
Official Symbol: EIF4ENIF1
Accession Number(s): NP_062817.2; NP_001157973.1; NP_001157974.1
Human GeneID(s): 56478
Non-Human GeneID(s): 74203 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms. Variants (NP_062817.2; NP_001157973.1) encode the same protein.

Immunogen

Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2; NP_001157973.1; NP_001157974.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5µg/ml
IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Background Reference

Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N.
A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.
EMBO J. 2000 Jun 15;19(12):3142-56.
PMID: 10856257

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