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Code Name Applications Availability Size Species reactivity Price Grade  
EB06443 Goat Anti-58KGolgi protein(Internal)/FTCD Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $315.00 Elite

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EB06443 1
EB06443 staining (0.01µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LC1 autoantigen, formimidoyltransferase cyclodeaminase, formiminotransferase cyclodeaminase, LCHC1, FTCD
Official Symbol: FTCD
Accession Number(s): NP_006648.1; NP_996848.1
Human GeneID(s): 10841
Important Comments: Variants (NP_006648.1; NP_996848.1) encode the same protein.

Immunogen

Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1; NP_996848.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Hagiwara H, Tajika Y, Matsuzaki T, Suzuki T, Aoki T, Takata K.
Localization of Golgi 58K protein formiminotransferase cyclodeaminase) to the centrosome.
Histochem Cell Biol. 2006 Aug;126(2):251-9. Epub 2006 Mar 14.
PMID: 16534631

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