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Goat Anti-ABCB9 / TAPL Antibody (EB05736)

Related Products: EBP05736 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05736 Goat Anti-ABCB9 / TAPL Antibody ELISA, IHC Yes 100µg specific antibody in 200µl Human $300.00 Elite

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

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San Diego
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Toll Free Tel: 1-800-550-3033
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EB05736 1
EB05736 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EST122234, KIAA1520, TAPL, ATP-binding cassette, sub-family B (MDR/TAP), member 9, ABCB9
Official Symbol: ABCB9
Accession Number(s): NP_062570.1; NP_062571.1; NP_982269.1
Human GeneID(s): 23457
Important Comments: This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1; NP_982269.1) encode the same isoform.

Immunogen

Peptide with sequence C-GHNEPVANGSHKA, from the C Terminus of the protein sequence according to NP_062570.1; NP_062571.1; NP_982269.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Testis shows vesiculate cytoplasm staining of primary spermatocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Zhang F, Zhang W, Liu L, Fisher CL, Hui D, Childs S, Dorovini-Zis K, Ling V.
Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes.
J Biol Chem. 2000 Jul 28;275(30):23287-94.
PMID: 10748049

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