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Goat Anti-Adenosine A2b Receptor Antibody (EB06936)

Related Products: EBP06936 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06936 Goat Anti-Adenosine A2b Receptor Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06936 1
EB06936 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: netrin-1 receptor, ADORA2, HGNC:264, ADORA2B, adenosine A2b receptor
Official Symbol: ADORA2B
Accession Number(s): NP_000667.1
Human GeneID(s): 136

Immunogen

Peptide with sequence CQADVKSGNGQ, from the C Terminus of the protein sequence according to NP_000667.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Colon, Lung and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules . Recommended concentration, 3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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