Change country
Home

Goat Anti-ALS2CR1 / NIF3L1 Antibody (EB05869)

Related Products: EBP05869 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05869 Goat Anti-ALS2CR1 / NIF3L1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05869 1
EB05869 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05869 2
EB05869 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NIF3L1, ALS2CR1, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), CALS-7, MDS015, NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1, NIF3 NGG1 interacting factor 3-like 1, OTTHUMP00000206190, OTTHUMP00000206191, OTTHUMP00000206192
Official Symbol: NIF3L1
Accession Number(s): NP_001129511.1; NP_068596.2
Human GeneID(s): 60491
Non-Human GeneID(s): 65102 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.

Immunogen

Peptide with sequence C-ILSETDRDPLQVV, from the C Terminus of the protein sequence according to NP_001129511.1; NP_068596.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39kDa according to NP_068596]. Recommended for use at 0.2-1µg/ml.
IHC: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Product Reviews

Please login to review this product.