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Goat Anti-ALS2CR1 / NIF3L1 Antibody (EB05869)

Related Products: EBP05869 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05869 Goat Anti-ALS2CR1 / NIF3L1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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EB05869 1
EB05869 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05869 2
EB05869 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000206192, OTTHUMP00000206191, OTTHUMP00000206190, NIF3 NGG1 interacting factor 3-like 1, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1, NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1, MDS015, CALS-7, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), ALS2CR1, NIF3L1
Official Symbol: NIF3L1
Accession Number(s): NP_001129511.1; NP_068596.2
Human GeneID(s): 60491
Non-Human GeneID(s): 65102 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.

Immunogen

Peptide with sequence C-ILSETDRDPLQVV, from the C Terminus of the protein sequence according to NP_001129511.1; NP_068596.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39kDa according to NP_068596]. Recommended for use at 0.2-1µg/ml.
IHC: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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