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Goat Anti-ARP1 homolog A Antibody (EB06349)

Related Products: EBP06349 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06349 Goat Anti-ARP1 homolog A Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Dog, Pig $315.00 Elite

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EB06349 1
EB06349 staining (2µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARP1 actin-related protein 1 homolog A, centractin alpha, FLJ55002, FLJ52800, FLJ52695, CTRN1, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha), centrosome-associated actin homolog, ARP1, yeast homolog A, centractin alpha, actin-RPV, ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), ARP1, ACTR1A
Official Symbol: ACTR1A
Accession Number(s): NP_005727.1
Human GeneID(s): 10121
Non-Human GeneID(s): 54130 (mouse)
Important Comments: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

Immunogen

Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Pig

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