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Goat Anti-ARP1 homolog B Antibody (EB06350)

Related Products: EBP06350 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06350 Goat Anti-ARP1 homolog B Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $300.00 Elite

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EB06350 1
EB06350 staining (1µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06350 2
EB06350 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARP1 actin-related protein 1 homolog B, centractin beta, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1, yeast homolog B, centractin beta, PC3, ARP1 actin-related protein 1 homolog B, centractin beta (yeast), CTRN2, ARP1B, ACTR1B
Official Symbol: ACTR1B
Accession Number(s): NP_005726.1
Human GeneID(s): 10120
Non-Human GeneID(s): 226977 (mouse)
Important Comments: This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).

Immunogen

Peptide with sequence C-YEEDGSRAIHRKT, from the C Terminus of the protein sequence according to NP_005726.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2µg/ml.
IHC: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Background Reference

Clark SW, Staub O, Clark IB, Holzbaur EL, Paschal BM, Vallee RB, Meyer DI.
Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.
Mol Biol Cell. 1994 Dec;5(12):1301-10.
PMID: 7696711

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