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Goat Anti-Asporin / ASPN Antibody (EB06670)

Related Products: EBP06670 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06670 Goat Anti-Asporin / ASPN Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB06670 1
EB06670 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06670 2
EB06670 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.
Click here to download PDF datasheet.

Target Protein

Principal Names: asporin proteoglycan, OS3, asporin, periodontal ligament associated protein 1, small leucine-rich protein 1C, asporin (LRR class 1), FLJ20129, SLRR1C, PLAP1, ASPN
Official Symbol: ASPN
Accession Number(s): NP_060150.3
Human GeneID(s): 54829

Immunogen

Peptide with sequence C-IHENKVKKIQKDT, from the internal region of the protein sequence according to NP_060150.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 1-3µg/ml.

Immunofluorescence: In ATDC5 cell line signal observed in cytoplasm and on the cell surface. Data gratefully received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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