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Code Name Applications Availability Size Species reactivity Price Grade  
EB05175 Goat Anti-BAIAP2 (isoform 3) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Dog, Cow $315.00 Elite

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EB05175 1
EB05175 staining (1µg/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: insulin receptor substrate p53/p58, insulin receptor substrate p53, fas ligand-associated factor 3, brain-specific angiogenesis inhibitor 1-associated protein 2, FLAF3, BAI1-associated protein 2, IRSP53, BAP2, BAIAP2
Official Symbol: BAIAP2
Accession Number(s): NP_006331.1
Human GeneID(s): 10458
Important Comments: This antibody will recognise only one of three reported isoforms (NP_006331.1).

Immunogen

Peptide with sequence C-SMSSADVEVARF, from the C Terminus of the protein sequence according to NP_006331.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4kDa according to NP_006331.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 150-170kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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