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Goat Anti-BCL2 Antibody (EB06444)

Related Products: EBP06444 - Immunizing Peptide, Goat Anti-BCL2 Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06444 Goat Anti-BCL2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB06444 1
EB06444 staining (1µg/ml) of Human Lymph Node lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000163680, B-cell lymphoma protein 2, B-cell CLL/lymphoma 2, Bcl-2, BCL2
Official Symbol: BCL2
Accession Number(s): NP_000624.2; NP_000648.2
Human GeneID(s): 596
Non-Human GeneID(s): 12043 (mouse), 24224 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000624; NP_000648)

Immunogen

Peptide with sequence AGRTGYDNREIVMKYC, from the N Terminus of the protein sequence according to NP_000624.2; NP_000648.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25 kDa band observed in Human Lymph node lysates (predicted MW of 26kDa according to NP_000624, alpha isoform). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Yu XQ, Xue CC, Zhou ZW, Li CG, Zhou SF.
Tanshinone IIB, a primary active constituent from Salvia miltiorrhiza, exerts neuroprotective effect via inhibition of neuronal apoptosis in vitro.
Phytother Res. 2008 Jun;22(6):846-50.
PMID: 18389485

The goat polyclonal antibody used in this paper was manufactured by us:
Yu XQ, Xue CC, Zhou ZW, Li CG, Du YM, Liang J, Zhou SF.
In vitro and in vivo neuroprotective effect and mechanisms of glabridin, a major active isoflavan from Glycyrrhiza glabra (licorice).
Life Sci. 2008 Jan 2;82(1-2):68-78. Epub 2007 Nov 7.
PMID: 18048062

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