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Goat Anti-BERP / RNF22 Antibody (EB05868)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05868 Goat Anti-BERP / RNF22 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05868 1
EB05868 staining (0.05µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05868 2
HEK293 overexpressing TRIM3 (RC211928) and probed with EB05868 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ16135, tripartite motif protein TRIM3, brain expressed ring finger, ring finger protein 22, RNF97, HAC1, tripartite motif-containing 3, TRIM3, RNF22, BERP, TRIM3
Official Symbol: TRIM3
Accession Number(s): NP_006449.2; NP_150594.2
Human GeneID(s): 10612
Non-Human GeneID(s): 55992 (mouse), 83616 (rat)
Important Comments: Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.

Immunogen

Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2]. Recommended for use at 0.1-1µg/ml.

Please note that the HEK cell experiments were performed using a previous batch.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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