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Goat Anti-BPOZ / ABTB1 Antibody (EB05200)

Related Products: EBP05200 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05200 Goat Anti-BPOZ / ABTB1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB05200 1
EB05200 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05200 2
EB05200 (3.8µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PP2259, Btb3, elongation factor 1A binding protein, ankyrin repeat and BTB (POZ) domain containing 1, MGC20585, EF1ABP, BPOZ, ABTB1
Official Symbol: ABTB1
Accession Number(s): NP_115937.1; NP_742024.1; NP_742025.1
Human GeneID(s): 80325
Non-Human GeneID(s): 80283 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).

Immunogen

Peptide with sequence RALEDLLVSIGLDC, from the C Terminus of the protein sequence according to NP_115937.1; NP_742024.1; NP_742025.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed in Human Heart lysates (calculated MW of 37.6kDa according to NP_115937.1). Recommended for use at 1-3µg/ml.
IHC: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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