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Code Name Applications Availability Size Tested species Price Grade  
EB06945 Goat Anti-Cannabinoid Receptor 1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $315.00 Elite

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ARP American Research Products, Inc
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EB06945 1
EB06945 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: cannabinoid receptor 1 (brain), CNR1, HGNC:2159, CANN6, CB-R, CB1, CB1A, CB1K5, CNR, central cannabinoid receptor, RP1-23D17.1, CB1R, OTTHUMP00000016838, OTTHUMP00000016839, cannabinoid receptor 1
Official Symbol: CNR1
Accession Number(s): NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1
Human GeneID(s): 1268
Non-Human GeneID(s): 12801 (mouse), 25248 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1).

Immunogen

Peptide with sequence C-HTSEDGKVQVTRPDQ, from the internal region of the protein sequence according to NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band observed in Rat Brain lysates (calculated MW of 52.7kDa according to Rat NP_036916.1 and 52.9kDa according to Human NP_057167.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Human: Fügedi G et al, BMC Pregnancy Childbirth. 2014 Dec 2;14:395. PMID: 25444073.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Human:
Fügedi G, Molnár M, Rigó J Jr, Schönléber J, Kovalszky I, Molvarec A.
Increased placental expression of cannabinoid receptor 1 in preeclampsia: an observational study.
BMC Pregnancy Childbirth. 2014 Dec 2;14:395
PMID: 25444073

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