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Goat Anti-Cannabinoid Receptor 2 Antibody (EB06946)

Related Products: EBP06946 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06946 Goat Anti-Cannabinoid Receptor 2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06946 1
EB06946 (1.5µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: cannabinoid receptor 2 (macrophage), HGNC:2160, CNR3, CB2, CX5, OTTHUMP00000044841, CNR2, RP11-4M23.1, testis-dominant CNR2 isoform CB2
Official Symbol: CNR2
Accession Number(s): NP_001832.1
Human GeneID(s): 1269

Immunogen

Peptide with sequence C-TETEADGKITPWPD, from the C Terminus of the protein sequence according to NP_001832.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 46.2kDa according to NP_001832.1). This molecular weight is routinely observed by other sources. Primary incubation was 1 hour. Recommended concentration: 1.5-4.5µg/ml. This antibody has been successfully used in WB on Human: Fügedi G et al. (2014) PMID: 25444073.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot on Human:
Fügedi G, Molnár M, Rigó J Jr, Schönléber J, Kovalszky I, Molvarec A.
Increased placental expression of cannabinoid receptor 1 in preeclampsia: an observational study.
BMC Pregnancy Childbirth. 2014 Dec 2;14:395
PMID: 25444073

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