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Goat Anti-CARD6 Antibody (EB05922)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05922 | Goat Anti-CARD6 Antibody | ELISA, IHC |  |
100µg specific antibody in 200µl | Human | $300.00 |  |
|
Ordering Information forOrder direct from Everest
$35.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:
In stock orders received by 09.30 PST / 12.30 EST on Monday through Thursday are shipped the same day. OR
Order from our local distributorAxxora, LLC6181 Cornerstone Court East Suite 103, Tel: 858-550-8830 |
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Target Protein
Principal Names: CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation, CINCIN1, caspase recruitment domain protein 6, caspase recruitment domain family, member 6, CARD6Official Symbol: CARD6
Accession Number(s): NP_115976.2
Human GeneID(s): 84674
Immunogen
Peptide with sequence ATESTPSEIIERER-C, from the N Terminus of the protein sequence according to NP_115976.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
IHC: In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Background Reference
Stehlik C, Hayashi H, Pio F, Godzik A, Reed JC.
CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways.
J Biol Chem. 2003 May 29 [Epub ahead of print]
PMID: 12775719