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Goat Anti-CBX3 / HP1 Gamma Antibody (EB06959)

Related Products: EBP06959 - Immunizing Peptide, Goat Anti-CBX3 / HP1 Gamma Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06959 Goat Anti-CBX3 / HP1 Gamma Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $300.00 Elite

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CA 92121-4727
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Toll Free Tel: 1-800-550-3033
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EB06959 1
EB06959 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06959 2
EB06959 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: heterochromatin-like protein 1, heterochromatin protein HP1 gamma, chromobox homolog 3 (Drosophila HP1 gamma), chromobox homolog 3, HP1 gamma homolog, HP1Hs-gamma, HECH, HGNC:1553, chromobox homolog 3 (HP1 gamma homolog, Drosophila), HP1-GAMMA, CBX3
Official Symbol: CBX3
Accession Number(s): NP_009207.2; NP_057671.2
Human GeneID(s): 11335
Non-Human GeneID(s): 12417 (mouse), 297093 (rat)
Important Comments: No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.

Immunogen

Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2; NP_057671.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 20kDa band observed in lysates of A431, HeLa and HepG2. (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 µg/ml.
IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Background Reference

Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.
Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin.
Mol Cell. 2005 Aug 5;19(3):381-391.
PMID: 16061184

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