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Goat Anti-CD32 / FCGR2B Antibody (EB06695)

Code Name Applications Availability Size Tested species Price Grade  
EB06695 Goat Anti-CD32 / FCGR2B Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06695 1
EB06695 (1µg/ml) staining of Daudi cell lysate (RIPA buffer, (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06695 2
EB06695 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06695 3
EB06695 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of IgG, low affinity IIb, receptor for (CD32), Fc gamma RIIb, Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), CD32B, Fc fragment of IgG, low affinity IIb, receptor
Official Symbol: FCGR2B
Accession Number(s): NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1
Human GeneID(s): 2213
Important Comments: This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).

Immunogen

Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 34.0kDa according to NP_003992.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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