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Goat Anti-CENTG3 / MRIP1 Antibody (EB06127)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06127 | Goat Anti-CENTG3 / MRIP1 Antibody | ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $300.00 |  |
|
Ordering Information forOrder direct from Everest
$35.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:
In stock orders received by 09.30 PST / 12.30 EST on Monday through Thursday are shipped the same day. OR
Order from our local distributorAxxora, LLC6181 Cornerstone Court East Suite 103, Tel: 858-550-8830 |
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Target Protein
Principal Names: centaurin, gamma 3, CRMP (collapsin response mediator protein) associated, FLJ34452, FLJ16146, CRAG, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, MRIP-1 protein, MRIP-1, centaurin, gamma 3, MRIP1, CENTG3, AGAP3Official Symbol: AGAP3
Accession Number(s): NP_114152.3
Human GeneID(s): 116988
Non-Human GeneID(s): 213990 (mouse)
Immunogen
Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat
Background Reference
Surks HK, Richards CT, Mendelsohn ME.
Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA.
J Biol Chem. 2003 Dec 19;278(51):51484-93. Epub 2003 Sep 23.
PMID: 14506264