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Goat Anti-CXXC4 Antibody (EB09211)

Related Products: EBP09211 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09211 Goat Anti-CXXC4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09211 1
EB09211 (3.8µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CXXC4, CXXC finger 4, IDAX, MGC149872, Dvl-binding protein IDAX (inhibition of the Dvl and Axin complex), inhibition of the Dvl and Axin complex
Official Symbol: CXXC4
Accession Number(s): NP_079488.1
Human GeneID(s): 80319
Non-Human GeneID(s): 319478 (mouse)

Immunogen

Peptide with sequence C-GTSLERTPVPSAEA, from the C-Terminus (near) of the protein sequence according to NP_079488.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human Brain (Cerebellum, Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Breast shows cystoplasm staining in myoepithelial cells. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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