Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-DDX5 / p68 RNA helicase Antibody (EB06310)

Related Products: EBP06310 - Immunizing Peptide, Goat Anti-DDX5 / p68 RNA helicase Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06310 Goat Anti-DDX5 / p68 RNA helicase Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

Ordering Information for

Order direct from Everest

$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB06310 1
EB06310 staining (0.5µg/ml) of A431 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06310 2
EB06310 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DEAD box-5, ATP-dependent RNA helicase DDX5, DKFZp686J01190, DKFZp434E109, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD/H box-5 (RNA helicase, 68kD), RNA-dependent ATPase, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), HUMP68, G17P1, HLR1, P68, DDX5
Official Symbol: DDX5
Accession Number(s): NP_004387.1
Human GeneID(s): 1655
Non-Human GeneID(s): 13207 (mouse)

Immunogen

Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3µg/ml.
IHC: In paraffin embedded Human Prostate shows nuclear staining of the secretory cells in the glands. Recommended concentration, 2-5µg/ml.

Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Fukuda T, Yamagata K, Fujiyama S, Matsumoto T, Koshida I, Yoshimura K, Mihara M, Naitou M, Endoh H, Nakamura T, Akimoto C, Yamamoto Y, Katagiri T, Foulds C, Takezawa S, Kitagawa H, Takeyama K, O'Malley BW, Kato S.
DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs.
Nat Cell Biol. 2007 May;9(5):604-11.
PMID: 17435748

Product Reviews

Please login to review this product.