Change country

Goat Anti-Doublecortin (mouse) Antibody (EB11639)

Code Name Applications Availability Size Tested species Price Grade  
EB11639 Goat Anti-Doublecortin (mouse) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11639 1
EB11639 (0.5µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11639 2
EB11639 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: Dbct, Dcx, doublecortex, doublecortin, doublin, lissencephalin-X, lissencephaly, X-linked (doublecortin), lis-X, neuronal migration protein doublecortin, OTTMUSP00000020394, OTTMUSP00000020395, OTTMUSP00000020396, OTTMUSP00000020397
Official Symbol: Dcx
Accession Number(s): NP_001103692.1; NP_001103694.1; NP_034155.2
Human GeneID(s): 1641
Non-Human GeneID(s): 13193 (mouse), 84394 (rat)
Important Comments: This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1

Immunogen

Peptide with sequence C-PKASPTPQKTSAKS, from the internal region of the protein sequence according to NP_001103692.1; NP_001103694.1; NP_034155.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to NP_001103692.1). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5µg/ml

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Product Reviews

Please login to review this product.