Change country
Home

Goat Anti-Dynactin Antibody (EB05639)

Code Name Applications Availability Size Tested species Price Grade  
EB05639 Goat Anti-Dynactin Antibody Pep-ELISA, WB, FC, IF, ICC, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05639 1
EB05639 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05639 2
EB05639 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05639 3
EB05639 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05639 4
EB05639 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05639 5
EB05639 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog
Official Symbol: DCTN1
Accession Number(s): NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1
Human GeneID(s): 1639
Non-Human GeneID(s): 13191 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2). Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.
IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4µg/ml. A customer found positive results in IHC on Human HeLa cells.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse

Product Reviews

Please login to review this product.