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Goat Anti-Dynactin Antibody (EB05639)

Code Name Applications Availability Size Tested species Price Grade  
EB05639 Goat Anti-Dynactin Antibody Pep-ELISA, WB, IHC, ICC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
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EB05639 1
EB05639 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05639 2
EB05639 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog
Official Symbol: DCTN1
Accession Number(s): NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1
Human GeneID(s): 1639
Non-Human GeneID(s): 13191 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).


Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150kDa band observed in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2µg/ml. Primary incubated for 1 hour. A customer found positive results in WB on Mouse embryonic fibroblasts (MEF cells). A customer found positive results in WB on Human HeLa cells. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.
IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4µg/ml. A customer found positive results in IHC on Human HeLa cells.

Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody was successfully used in the following paper:
Bupha-Intr T, Haizlip KM, Janssen PM.
Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.
Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.
PMID: 19136602

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