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Goat Anti-Dynactin Antibody (EB05639)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05639 Goat Anti-Dynactin Antibody Pep-ELISA, WB, IHC, ICC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB05639 1
EB05639 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05639 2
EB05639 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCTN1 antibody, dynactin 1 (p150, glued homolog, Drosophila) antibody, P135 antibody, DP-150 antibody, DAP-150 antibody, p150-glued antibody, p150, Glued (Drosophila) homolog antibody, 150 kDa dynein-associated polypeptide antibody, dynactin 1 (p150, Glued (Drosophila) homolog) antibody, HMN7B antibody, OTTHUMP00000202491 antibody, dynactin 1 antibody, p150, Glued homolog antibody
Official Symbol: DCTN1
Accession Number(s): NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1
Human GeneID(s): 1639
Non-Human GeneID(s): 13191 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).

Immunogen

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150kDa band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2µg/ml. An anonymous customer found positive results in WB on Mouse embryonic fibroblasts (MEF cells). An anonymous customer found positive results in WB on Human HeLa cells.
IHC: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4µg/ml. An anonymous customer found positive results in IHC on Human HeLa cells.

Immunocytochemsitry: An anonymous customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

The goat polyclonal antibody used as described in this paper was manufactured by us:
Bupha-Intr T, Haizlip KM, Janssen PM.
Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.
Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.
PMID: 19136602

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