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Goat Anti-E2-EPF / UBE2S Antibody (EB05758)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05758 Goat Anti-E2-EPF / UBE2S Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $210.00 Aspiring

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

Target Protein

Principal Names: ubiquitin-protein ligase S, ubiquitin carrier protein S, EPF5, E2EPF, ubiquitin-conjugating enzyme E2S, UBE2S, ubiquitin-conjugating enzyme E2-24 kD, ubiquitin-protein ligase, ubiquitin carrier protein, E2-EPF
Official Symbol: UBE2S
Accession Number(s): NP_055316.2
Human GeneID(s): 27338
Non-Human GeneID(s): 77891 (mouse)

Immunogen

Peptide with sequence NSNVENLPPHIIR-C, from the N Terminus of the protein sequence according to NP_055316.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 35-40kDa band observed in Human Liver lysate at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 25kDa according to NP_055316. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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