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Goat Anti-FGFR1 Antibody (EB06649)

Related Products: EBP06649 - Immunizing Peptide, EBP11208 - Immunizing Peptide, Goat Anti-FGFR1 Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB06649 Goat Anti-FGFR1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Dog, Cow $315.00 Elite

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EB06649 1
EB06649 (4µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06649 2
EB06649 (0.3µg/ml) staining of human breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: soluble FGFR1 variant 2, soluble FGFR1, OTTHUMP00000190881, OGD, HBGFR, FLJ99988, FGFBR, CD331, fibroblast growth factor receptor 1, basic fibroblast growth factor receptor 1, heparin-binding growth factor receptor, fms-related tyrosine kinase-2, hydroxyaryl-protein kinase, FMS-like tyrosine kinase 2, protein-tyrosine kinase, tyrosylprotein kinase, N-sam tyrosine kinase, FLG protein, fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome), N-SAM, C-FGR, BFGFR, KAL2, FLT2, FLG, CEK, H5, H4, H3, H2, FGFR1
Official Symbol: FGFR1
Accession Number(s): NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1
Human GeneID(s): 2260
Important Comments: This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1).

Immunogen

Peptide with sequence CLPRHPAQLANGGLKR, from the C Terminus of the protein sequence according to NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in human breast lysates (calculated MW of 82.2kDa according to NP_075593, 81.9kDa according to NP_075594). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Small Intestine shows staining of the enterocytic cytoplasm. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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