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Code Name Applications Availability Size Tested species Price Grade  
EB06006 Goat Anti-FOXC1 (C Term) Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06006 1
EB06006 (0.3µg/ml) staining of HEK293 (A) Nuclear MCF7 (B) and Negative control Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06006 2
EB06006 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06006 3
EB06006 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06006 4
EB06006 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IRID1, FREAC3, iridogoniodysgenesis type 1, forkhead (Drosophila)-like 7, forkhead-related activator 3, Forkhead, drosophila, homolog-like 7, FREAC-3, RIEG3, forkhead/winged helix-like transcription factor 7, myeloid factor-delta
Official Symbol: FOXC1
Accession Number(s): NP_001444.2
Human GeneID(s): 2296
Non-Human GeneID(s): 17300 (mouse)

Immunogen

Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70-75kDa band observed in lysates of cell lines HEK293 and nuclear MCF7 (calculated MW of 56.8kDa according to NP_001444.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ul. Primary incubation 1 hour at room temperature. Negative Control: Daudi cell lysate.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. Chromatin immunoprecipitation: Customer successfully used this antibody in ChIP from human cell line. This data is from a previous batch, not on sale.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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