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Code Name Applications Availability Size Tested species Price Grade  
EB06006 Goat Anti-FOXC1 (C Term) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB06006 1
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with EB06006 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB06006 (0.5mg/ml) in Lane B. Detected by chemiluminescence.
EB06006 2
EB06006 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06006 3
EB06006 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IRID1, FREAC3, iridogoniodysgenesis type 1, forkhead (Drosophila)-like 7, forkhead-related activator 3, Forkhead, drosophila, homolog-like 7, FREAC-3, RIEG3, forkhead/winged helix-like transcription factor 7, myeloid factor-delta
Official Symbol: FOXC1
Accession Number(s): NP_001444.2
Human GeneID(s): 2296
Non-Human GeneID(s): 17300 (mouse)


Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1µg/ml. Primary incubations for 1 hour.

A customer found positive results in WB on Human breast cancer cells (T47D).

Chromatin immunoprecipitation: Customer successfully used this antibody in ChIP from human cell line. This data is from a previous batch, not on sale.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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