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Goat Anti-FOXC2 Antibody (EB06007)

Code Name Applications Availability Size Tested species Price Grade  
EB06007 Goat Anti-FOXC2 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06007 1
EB06007 (0.1µg/ml) staining of nuclear HEK293 (A), (0.3ug/ml) nuclear Jurkat (B) and (0.5ug/ml) negative control Human Pancreas (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06007 2
EB06007 (2µg/ml) staining of paraffin embedded Human Breast Cancer. Steamed antigen retrieval with Tris/EDTA buffer Ph 9, HRP-staining.
EB06007 3
EB06007 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14
Official Symbol: FOXC2
Accession Number(s): NP_005242.1
Human GeneID(s): 2303
Non-Human GeneID(s): 14234 (mouse)

Immunogen

Peptide with sequence RHAAPYSYDCTKY, from the C Terminus of the protein sequence according to NP_005242.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and Jurkat (calculated MW of 53.7kDa according to NP_005242.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.
IHC: In paraffin embedded Human Breast Cancer shows nuclear staining with some weak cytoplasm staining. Recommended concentration: 2-4µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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