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Goat Anti-FOXG1 / BF2 Antibody (EB05608)

Code Name Applications Availability Size Tested species Price Grade  
EB05608 Goat Anti-FOXG1 / BF2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05608 1
EB05608 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: forkhead box G1, BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN, OTTHUMP00000178764, brain factor 2, forkhead box G1A, forkhead box G1B, forkhead box G1C, forkhead, drosophila, homolog-like 2, forkhead-like 1, forkhead-like 2, forkhead-like 3, forkhead-like 4, oncogene QIN
Official Symbol: FOXG1
Accession Number(s): NP_005240.3
Human GeneID(s): 2290
Non-Human GeneID(s): 15228 (mouse), 24370 (rat)

Immunogen

Peptide with sequence C-HQNQGSSSNPLIH, from the C Terminus of the protein sequence according to NP_005240.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A)). Recommended concentration: 0.3-1.0µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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