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Goat Anti-galectin-1 (mouse) Antibody (EB10777)

Related Products: EBP10777 - Immunizing Peptide, EBP10778 - Immunizing Peptide, Goat Anti-galectin-1 (mouse) (aa100-112) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10777 Goat Anti-galectin-1 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10777 1
EB10777 (0.1µg/ml) staining of Mouse (A) and Rat (B) Pancreas lysates and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Lgals1, lectin, galactose binding, soluble 1, AA410090, Gal-1, Galbp, L-14.5, L14, Lect14, galectin-1, S-Lac lectin 1, beta-galactoside binding protein, beta-galactoside-binding lectin L-14-I, galaptin, galectin-1, lactose-binding lectin 1
Official Symbol: Lgals1
Accession Number(s): NP_032521.1
Human GeneID(s): 3956
Non-Human GeneID(s): 16852 (mouse), 56646 (rat)

Immunogen

Peptide with sequence KPGECLKVRGEVASD, from the N Terminus (near) of the protein sequence according to NP_032521.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 15kDa band observed in lysates of Mouse and Rat Pancreas and of cell line NIH3T3 (calculated MW of 14.9kDa according to NP_032521.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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