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Goat Anti-Glycophorin A Antibody - Trial (EB06947-T)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06947-T Goat Anti-Glycophorin A Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

TestShot
EB06947-T 1
EB06947 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06947-T 2
EB06947 (1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: glycophorin MiIII, glycophorin A (MN blood group), HGpMiIII, GpMiIII, CD235a, recombinant glycophorin A-B Miltenberger-DR, recombinant MiDR, glycophorin Sta type C, glycophorin SAT, glycophorin MiX, glycophorin MiV, glycophorin MiI, glycophorin Erik, glycophorin A, GPA, glycophorin A, erythroid-lineage-specific membrane sialoglycoprotein, Mi.V glycoprotein (24 AA), MNS, MN, HGpSta(C), HGpMiXI, HGpMiX, HGpMiV, GPSAT, GPErik, GPA, GYPA, HGNC:4702, glycophorin A (includes MN blood group)
Official Symbol: GYPA
Accession Number(s): NP_002090.2
Human GeneID(s): 2993

Immunogen

Peptide with sequence C-SVEIENPETSDQ, from the C Terminus of the protein sequence according to NP_002090.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 18kDa band observed in human liver lysates (calculated MW of 16.4kDa according to NP_002090.2). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Kidney shows bright staining of erythrocytes. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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