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Goat Anti-GNAI2 (aa289-299) Antibody (EB11980)

Code Name Applications Availability Size Tested species Price Grade  
EB11980 Goat Anti-GNAI2 (aa289-299) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB11980 1
EB11980 (2µg/ml) staining of Human Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11980 2
EB11980 (5µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11980 3
EB11980 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GNAI2, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1, GTP-binding regulatory protein Gi alpha-2 chain, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide-binding protein G(i) subunit alpha-2, guanine nucleotide-binding protein G(i), alpha-2 subunit
Official Symbol: GNAI2
Accession Number(s): NP_002061.1; NP_001159897.1
Human GeneID(s): 2771
Non-Human GeneID(s): 14678 (mouse), 81664 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002061.1; NP_001159897.1).

Immunogen

Peptide with sequence C-PEYTGANKYDE, from the internal region of the protein sequence according to NP_002061.1; NP_001159897.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 40.5kDa according to NP_002061.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thyroid and Tonsil. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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