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Goat Anti-GPS1 / COPS1 Antibody (EB05952)

Related Products: EBP05952 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05952 Goat Anti-GPS1 / COPS1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05952 1
EB05952 staining (0.5µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
EB05952 2
EB05952 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05952 3
EB05952 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05952 4
EB05952 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GPS1, COPS1, G protein pathway suppressor 1, Arabidopsis FUS6/COP11 homolog, CSN1, MGC71287
Official Symbol: GPS1
Accession Number(s): NP_997657.1; NP_004118.3
Human GeneID(s): 2873
Non-Human GeneID(s): 209318 (mouse), 117039 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_997657.1) and isoform 2 (NP_004118.3).

Immunogen

Peptide with sequence C-PANSQSRMSTNM, from the C Terminus of the protein sequence according to NP_997657.1; NP_004118.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Human Testis lysate (predicted MW of 58kDa according to NP_004118). Recommended for use at 0.5µg/ml. Primary incubation was for 1 hour.
IHC: Paraffin embedded Human Prostate, Testis and Tonsil. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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