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Goat Anti-GULP1 / CED6 Antibody (EB05447)

Related Products: EBP05447 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05447 Goat Anti-GULP1 / CED6 Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $300.00 Elite

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EB05447 1
EB05447 staining (0.2µg/ml) of Human Placenta lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05447 2
EB05447 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: engulfment adapter protein, PTB domain adaptor protein CED-6, OTTHUMP00000205911, OTTHUMP00000205909, OTTHUMP00000205908, FLJ31156, CED6, GULP, engulfment adaptor PTB domain containing 1, CED-6 protein, GULP1, CED-6
Official Symbol: GULP1
Accession Number(s): NP_057399.1
Human GeneID(s): 51454
Non-Human GeneID(s): 70676 (mouse)

Immunogen

Peptide with sequence EGTVFCLDPLDSRC, from the C Terminus of the protein sequence according to NP_057399.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6µg/ml. An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration, 5-10 µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Background Reference

Su HP, Nakada-Tsukui K, Tosello-Trampont AC, Li Y, Bu G, Henson PM, Ravichandran KS.
Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP).
J Biol Chem. 2002 Apr 5;277(14):11772-9. Epub 2001 Nov 29.
PMID: 11729193

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