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Goat Anti-H11 / HSP22 Antibody (EB05677)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05677 Goat Anti-H11 / HSP22 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB05677 1
EB05677 staining (1µg/ml) of human muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: heat shock protein beta-8, E2-induced gene 1, spinal muscular atrophy, distal, adult, autosomal dominant, small stress protein-like protein HSP22, protein kinase H11, hereditary motor neuropathy, distal, heat shock 27kDa protein 8, Charcot-Marie-Tooth disease, spinal, HSP22, HMN2A, HMN2, H11, E2IG1, DHMN2, CMT2L, heat shock 22kDa protein 8, HSPB8
Official Symbol: HSPB8
Accession Number(s): NP_055180.1
Human GeneID(s): 26353

Immunogen

Peptide with sequence NELPQDSQEVTCT, from the C Terminus of the protein sequence according to NP_055180.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 20-25kDa band seen in human skeletal muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Wilhelmus MM, Boelens WC, Otte-Höller I, Kamps B, Kusters B, Maat-Schieman ML, de Waal RM, Verbeek MM.
Small heat shock protein HspB8: its distribution in Alzheimer's disease brains and its inhibition of amyloid-beta protein aggregation and cerebrovascular amyloid-beta toxicity.
Acta Neuropathol. 2006 Feb;111(2):139-49.
PMID: 16485107

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