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Goat Anti-HYPB / SETD2 (internal region) Antibody (EB08118)

Code Name Applications Availability Size Tested species Price Grade  
EB08118 Goat Anti-HYPB / SETD2 (internal region) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB08118 1
EB08118 (0.5µg/ml) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SETD2, SET domain containing 2, FLJ16420, FLJ22472, FLJ23184, FLJ45883, HIF-1, HSPC069, HYPB, KIAA1732, huntingtin interacting protein 1, huntingtin interacting protein B
Official Symbol: SETD2
Accession Number(s): NP_054878.5; NP_001336299.1
Human GeneID(s): 29072
Non-Human GeneID(s): 235626 (mouse)

Immunogen

Peptide with sequence C-ERDPDKQTQNKE, from the internal region of the protein sequence according to NP_054878.5; NP_001336299.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 290kDa band observed in lysates of mouse fibroblasts. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231kDa according to NP_054878.3). Data obtained from anonymous customer and obtained from a previous batch (from a different goat). The Ponceau red demonstrates proper protein loading. Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Yi X, Tao Y, Lin X, Dai Y, Yang T, Yue X, Jiang X, Li X, Jiang DS, Andrade KC, Chang J.
Histone methyltransferase Setd2 is critical for the proliferation and differentiation of myoblasts.
Biochim Biophys Acta. 2017 Apr;1864(4):697-707.
PMID: 28130125

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