Change country
Home

Goat Anti-IFNGR1 (aa181-193) Antibody (EB12101)

Related Products: EBP12101 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12101 Goat Anti-IFNGR1 (aa181-193) Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12101 1
EB12101 (1µg/ml) staining of K562 (A) Caco-2 (B) and HepG2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12101 2
EB12101 (2µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12101 3
EB12101 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12101 4
EB12101 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12101 5
EB12101 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: IFNGR1, interferon gamma receptor 1, CD119, IFNGR, AVP, type 2, CD119 antigen, CDw119, IFN-gamma receptor 1, IFN-gamma-R1, antiviral protein, type 2, immune interferon receptor 1, interferon-gamma receptor alpha chain
Official Symbol: IFNGR1
Accession Number(s): NP_000407.1; NP_001350455.1; NP_001350456.1
Human GeneID(s): 3459

Immunogen

Peptide with sequence C-SEIQYKILTQKED, from the internal region of the protein sequence according to NP_000407.1; NP_001350455.1; NP_001350456.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 70kDa band observed in lysates of cell lines K562, Caco-2 and HepG2, and approx. 50kDa in Human Spleen lysates (calculated MW of 50.1kDa according to NP_001350456.1). The 70kDa observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the membranes of Caco-2 and THP-1 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.