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Goat Anti-Kalirin (isoform 2) Antibody (EB06143)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06143 Goat Anti-Kalirin (isoform 2) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB06143 1
EB06143 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KALRN antibody, kalirin, RhoGEF kinase antibody, FLJ16443 antibody, HAPIP antibody, TRAD antibody, duo antibody, huntingtin-associated protein interacting protein (duo) antibody, serine/threonine kinase with Dbl- and pleckstrin homology domains antibody, hCG_2022551 antibody, DUET antibody, DUO antibody, FLJ12332 antibody, FLJ18196 antibody, FLJ18623 antibody
Official Symbol: KALRN
Accession Number(s): NP_003938.1
Human GeneID(s): 8997
Non-Human GeneID(s): 545156 (mouse), 84009 (rat)
Important Comments: This antibody is expected to recognize isoform 2 (NP_003938.1).

Immunogen

Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 200kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates (calculated MW of 192kDa according to NP_003938.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: An anonymous customer found positive results in IHC on Mouse brain tissue (from previous batch, different goat).

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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