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Goat Anti-KPNA2 / IPOA1 Antibody (EB06233)

Related Products: EBP06233 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06233 Goat Anti-KPNA2 / IPOA1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB06233 1
EB06233 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06233 2
EB06233 staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.
Click here to download PDF datasheet.

Target Protein

Principal Names: pendulin, karyopherin alpha 2, importin-alpha-P1, importin alpha 2, importin alpha 1, RAG cohort 1, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), SRP1alpha, RCH1, IPOA1 QIP2, KPNA2
Official Symbol: KPNA2
Accession Number(s): NP_002257.1
Human GeneID(s): 3838
Non-Human GeneID(s): 16647 (mouse), 85245 (rat)

Immunogen

Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55-60kDa band observed in Hela lysates (predicted size of approx. 58kDa according to NP_002257.1). Recommended for use at 0.1-0.5µg/ml

Immunofluorescence: In 4% formaldahyde-fixed HeLa staining is mostly cytoplasmic, with some nuclear staining and clear nuclear pore staining.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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