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Goat Anti-LASP1 (N Terminus) Antibody (EB05251)

Related Products: EBP05251 - Immunizing Peptide, EBP08165 - Immunizing Peptide, Goat Anti-LASP1 (internal region) Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05251 Goat Anti-LASP1 (N Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig $315.00 Elite

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EB05251 1
EB05251 staining (0.1µg/ml) of Human Brain (Cerebellum) extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LIM and SH3 protein, Lasp-1, MLN50, LIM and SH3 protein 1, LASP1
Official Symbol: LASP1
Accession Number(s): NP_006139.1
Human GeneID(s): 3927
Non-Human GeneID(s): 16796 (mouse), 29278 (rat)

Immunogen

Peptide with sequence NPNCARCGKIVYP, from the N Terminus of the protein sequence according to NP_006139.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human Brain (Cerebellum) and Human Colon, Duodenum, Liver and Pancreas lysates (calculated MW of 29.7kDa according to NP_006139.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Li K, Hornshaw MP, van Minnen J, Smalla KH, Gundelfinger ED, Smit AB.
Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins.
J Proteome Res. 2005 May-Jun;4(3):725-33.
PMID: 15952719

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