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Goat Anti-MID2 / TRIM1 Antibody (EB05823)

Related Products: EBP05823 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05823 Goat Anti-MID2 / TRIM1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $315.00 Elite

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EB05823 1
EB05823 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05823 2
EB05823 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF60, FXY2, FLJ41813, FLJ37715, RP6-191P20.2, tripartite motif protein 1, midin 2, FXY2, midline 2, TRIM1, MID2
Official Symbol: MID2
Accession Number(s): NP_036348.2; NP_438112.2
Human GeneID(s): 11043
Non-Human GeneID(s): 23947 (mouse)
Important Comments: This antibody is expected to recognise both human isoforms of this protein.

Immunogen

Peptide with sequence QESPYVSGMKTCH, from the C Terminus of the protein sequence according to NP_036348.2; NP_438112.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in mouse heart lysates (calculated MW of 81.3kDa according to NP_036348.1 and 77.9kDa according to NP_438112.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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