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Goat Anti-MSX1 Antibody (EB06397)

Related Products: EBP06397 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06397 Goat Anti-MSX1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06397 1
EB06397 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06397 2
EB06397 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MSX1, HOX7, HYD1, msh homeo box homolog 1 (Drosophila), msh homeobox 1, STHAG1, homeobox 7, msh homeo box 1, msh homeobox homolog 1
Official Symbol: MSX1
Accession Number(s): NP_002439.2
Human GeneID(s): 4487
Non-Human GeneID(s): 17701 (mouse)

Immunogen

Peptide with sequence TSLPLGVKVEDS-C, from the N Terminus of the protein sequence according to NP_002439.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4µg/ml. Data obtained from a previous batch (different goat).

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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