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Goat Anti-MTMR9 (C Terminus) Antibody (EB05189)

Related Products: EBP05189 - Immunizing Peptide, EBP07179 - Immunizing Peptide, Goat Anti-MTMR9 (Internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05189 Goat Anti-MTMR9 (C Terminus) Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human, Rat $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05189 1
EB05189 (5ug/ml) staining of paraffin embedded Rat Spleen. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.
EB05189 2
EB05189 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05189 3
EB05189 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05189 4
EB05189 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MTMR9, myotubularin related protein 9, MTMR8, C8orf9, LIP-STYX, DKFZP434K171, DKFZp434K171, myotubularin related protein 8, MGC126672, myotubularin-related protein 9, myotubularin-related protein 9 variant
Official Symbol: MTMR9
Accession Number(s): NP_056273.2
Human GeneID(s): 66036

Immunogen

Peptide with sequence C-AELETEDGMQESP, from the C Terminus of the protein sequence according to NP_056273.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Rat Spleen shows staining of cytoplasm in select cells. Recommended concentration: 5-10µg/ml. This product has also been successfully used on Rat Hypothalamus.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Endoplasmic reticulum of A549 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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