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Goat Anti-Myosin IH Antibody (EB12108)

Related Products: EBP12108 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12108 Goat Anti-Myosin IH Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12108 1
EB12108 (0.1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12108 2
EB12108 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventional myosin-Ih
Official Symbol: Myo1H
Accession Number(s): NP_001158045.1
Human GeneID(s): 283446
Non-Human GeneID(s): 231646 (mouse), 687866 (rat)

Immunogen

Peptide with sequence C-EDQVYKDKNGQLR, from the C Terminus of the protein sequence according to NP_001158045.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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