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Goat Anti-NEIL1 / NEH1 Antibody (EB05859)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05859 Goat Anti-NEIL1 / NEH1 Antibody ELISA, IHC Yes 100µg specific antibody in 200µl Human $300.00 Elite

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EB05859 1
EB05859 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: nei endonuclease VIII-like 1, hFPG1, FPG1, endonuclease VIII, FLJ22402, NEI1, nei endonuclease VIII-like 1 (E. coli), NEH1, NEIL1
Official Symbol: NEIL1
Accession Number(s): NP_078884.2
Human GeneID(s): 79661

Immunogen

Peptide with sequence C-DIPSLEPEGTSAS, from the C Terminus of the protein sequence according to NP_078884.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Hazra TK, Izumi T, Boldogh I, Imhoff B, Kow YW, Jaruga P, Dizdaroglu M, Mitra S.
Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA.
Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3523-8.
PMID: 11904416

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