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Goat Anti-NMDA receptor 1 / GRIN1 Antibody (EB07341)

Code Name Applications Availability Size Tested species Price Grade  
EB07341 Goat Anti-NMDA receptor 1 / GRIN1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: info@arp1.com
www.arp1.com

EB07341 1
EB07341 (1µg/ml) staining of Human Amylgada (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRIN1, NMDA receptor 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1, NR1, N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta 1
Official Symbol: GRIN1
Accession Number(s): NP_000823.4; NP_067544.1; NP_015566.1
Human GeneID(s): 2902
Non-Human GeneID(s): 14810 (mouse), 24408 (rat)
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).

Immunogen

Peptide with sequence C-TQERVNNSNKKE, from the internal region of the protein sequence according to NP_000823.4; NP_067544.1; NP_015566.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 100kDa band observed in Human Brain (Amylgada) and Rat Brain lysates (calculated MW of 101kDa according to Human NP_067544.1 and Rat NP_001257534.1. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog. This batch is not expected to cross-react with Mouse in Western blot.

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