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Goat Anti-OCIL Antibody (EB09341)

Code Name Applications Availability Size Tested species Price Grade  
EB09341 Goat Anti-OCIL Antibody Pep-ELISA, WB, IF, FC, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09341 1
EB09341 (0.3µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09341 2
EB09341 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09341 3
EB09341 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB09341 4
EB09341 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09341 5
EB09341 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLEC2D, C-type lectin domain family 2, member D, CLAX, LLT1, OCIL, C-type lectin related f, C-type lectin superfamily 2, member D, lectin-like NK cell receptor, lectin-like transcript 1, osteoclast inhibitory lectin
Official Symbol: CLEC2D
Accession Number(s): NP_037401.1; NP_001004419.1; NP_001184246.1
Human GeneID(s): 29121
Important Comments: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).

Immunogen

Peptide with sequence C-LLRYKGPSDH, from the Internal region of the protein sequence according to NP_037401.1; NP_001004419.1; NP_001184246.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 30-35kDa band observed in lysates of histiocytic lymphoma cell line U937 (calculated MW of 22.3kDa according to NP_001004419.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil and Placenta. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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